Research Paper Volume 13, Issue 23 pp 25377—25392
Tartary buckwheat flavonoids relieve the tendency of mammary fibrosis induced by HFD during pregnancy and lactation
Figure 2. The effect of TBF on inflammatory levels induced by HFD during pregnancy and lactation. 1 g/L TBF was dissolved in 0.1% carboxymethylcellulose sodium solution and was ingested by mice by drinking water. Drinking water administration began on the first day of mating and stopped one week after delivery, and then mammary tissue was collected. After grinding and centrifugation, the supernatant of mice mammary tissue was obtained, and then the levels of MPO and pro-inflammatory cytokines in mammary tissue were determined. (A) Changes in MPO levels in mammary tissue; (B) Changes in IL-6 levels in mammary tissue; (C) Changes in IL-1β levels in mammary tissue; (D) Changes in the level of TNF-α in mammary tissue; The data error was based on SEM. Three independent repeated experiments were performed; #p < 0.01 vs. NT group; ∗∗p < 0.01 vs. HFD group.