Figure 6. The effect of TBF on fibrosis induced by HFD during pregnancy and lactation. 1 g/L TBF was dissolved in 0.1% carboxymethylcellulose sodium solution and was ingested by mice by drinking water. Drinking water administration began on the first day of mating and stopped one week after delivery, and then mammary tissue was collected. (A) Collagen 1, E-cadherin, α-SMA, Vimentin, β-Tublin protein bands; (B) Analysis of the relative density value of Collagen 1 protein; (C) Analysis of the relative density value of E-cadherin protein; (D) Analysis of relative density value of α-SMA protein; (E) Analysis of the relative density value of vimentin protein; β-Tubulin was used as a reference for protein control. The data error was based on SEM, three independent repeated experiments were performed; #p < 0.01 vs. NT group; ∗∗p < 0.01 vs. HFD group.