Research Paper Volume 13, Issue 23 pp 25393—25407
Functional rare variant in a C/EBP beta binding site in NINJ2 gene increases the risk of coronary artery disease
Figure 1. Assessment of the relationship between NINJ2 SNP rs34166160 and the expression level of NINJ2 mRNA by real-time RT-PCR analysis. Total RNA samples were isolated from blood samples (lymphocytes) of 9 subjects with an AC genotype and 80 subjects with a CC genotype. Real-time PCR analysis was used to analyze the relative expression level of NINJ2. A linear regression was used to compare the differences in the mean RQ values between different genotypes (AC and CC) of SNP rs34166160.