Research Paper Volume 13, Issue 23 pp 25393—25407
Functional rare variant in a C/EBP beta binding site in NINJ2 gene increases the risk of coronary artery disease
Figure 5. Increased expression of ninjurin2 induced by LPS stimulation can be blocked by knockdown of C/EBP beta. (A) The expression of C/EBP beta and ninjurin2 in HUVEC in the condition of LPS stimuli. HUVEC cells were plated on 24-well plates for 24 h and followed by stimulation with or without LPS (1 ug/mL) for another 24 h. Total protein extracts were prepared and blotted with the antibodies specific for NINJ2 or C/EBP beta. Three independent experiments were performed. Error bars represent standard deviation (SD). (B) The increased expression of ninjurin2 induced by LPS stimulation can be blocked by knockdown of C/EBP beta. The si RNA targeted to C/EBP beta were transfect into HUVECs for 24 h, and then LPS (1 ug/ml) stimulated for another 24 h.