Research Paper Volume 13, Issue 23 pp 25484—25495
Hsa_circ_0008259 modulates miR-21-5p and PDCD4 expression to restrain osteosarcoma progression
Figure 2. Circ_0008259 inhibits the proliferation and metastatic potential of OS cells and promoted the apoptosis. (A) qRT-PCR was employed to confirm that the circ_0008259 overexpression and circ_0008259 knockdown cell model was constructed successfully. (B) CCK-8 assay was used to detect the proliferation of OS cells (143B and HOS). (C) Transwell assay was used to detect the migration and invasion of OS cells (143B and HOS). (D) Flow cytometry was used to detect the apoptosis rate of OS cells (143B and HOS). **P < 0.01, and ***P < 0.001.