Research Paper Volume 13, Issue 23 pp 25484—25495
Hsa_circ_0008259 modulates miR-21-5p and PDCD4 expression to restrain osteosarcoma progression
Figure 5. Circ_0008259 represses OS progression via modulating miR-21-5p / PDCD4 axis. (A) 143B cells with circ_0008259 overexpression was transfected with miR-21-5p mimics and PDCD4 overexpression vector, and the expression of PDCD4 in OS cells was detected by Western blot assay. (B) CCK8 assay was used to detect the proliferation of 143B cells after transfection. (C, D) Transwell assay was used to detect the migration and invasion of 143B cells after transfection. (E) Flow cytometry was used to detect the apoptosis rate of 143B cells after transfection. **P < 0.01, and ***P < 0.001.