Research Paper Volume 14, Issue 10 pp 4305—4325
MRTF-A-mediated protection against amyloid-β-induced neuronal injury correlates with restoring autophagy via miR-1273g-3p/mTOR axis in Alzheimer models
Figure 7. The miR-1273g-3p/mTOR axis involved in MRTF-A against Aβ-induced autophagy impairment and neuronal apoptosis in SH-SY5Y cells. (A, B) LC3II, Beclin1 and p62 level was upregulated by miR-1273g-3p. (C, D) The autophagy flux analysis using Ad-mCherry-GFP-LC3B-tagged protein. (E, F) TUNEL staining for cell apoptosis. (G, H) LC3II, Beclin1 and p62 level was upregulated by MRTF-A. Data represent means ± SEM of 3 independent experiments. *P< 0.05, **P < 0.01 and ***P < 0.001 (one-way ANOVA, Tukey’s Multiple Comparison Test).