Research Paper Volume 14, Issue 6 pp 2574—2589

Figure 4. Effect of progesterone on myelin loss and axonal pathology. (A) Representative immunofluorescence staining images of MBP (green) and SMI32(red)in perihematomal region. Nuclei were counterstained with DAPI (blue). Bar=50μm. (B, C) Quantitative analyses of MBP and SMI32 relative fluorescent intensity in perihematomal region in each group. (D) Representative images of Luxol fast blue staining. Bar=50μm. (E) Quantitative analyses of positive stained myelin ratio in each group. (F) Representative Western bands showing the protein expression of NF200, GAP43, Nogo-A and MAG in perihematomal region. (G–J) Quantitative analysis of Western blots shows that the expression of NF200, GAP43, Nogo-A and MAG changes in each group. (K) Representative images of immunohistochemistry staining of GAP43 and MAG in perihematomal region. Bar=50μm. (L, M) Quantitative analyses of GAP43 and MAG positive expression in perihematomal region in each group. n = 6 animals per group. Data are expressed as the mean ± SEM; **P < 0.01 vs. sham; ^#P < 0.05 vs. ICH group; ^##P < 0.01 vs. ICH group. MBP: myelin basic protein; SMI32: SMI32: Stemberger Monoclonal Incorporated Antibody 32; GAP43: Growth associated protein-43; NF200: Neurofilament; Nogo-A: Neurite outgrowth inhibitor-A; MAG: Myelin Associated Glycoprotein.