Priority Research Paper Volume 14, Issue 6 pp 2442—2461

Regulation of mTOR complexes in long-lived growth hormone receptor knockout and Snell dwarf mice


Figure 4. mTORC1 or mTORC2 substrates are not regulated by DEPTOR or PRAS40 knockdown in GHRKO cells. (A) Representative immunoblots of the protein expression in GHRKO cells. (B) Quantification of the protein levels in a series of experiment as in (A). (C, D) Protein expression of mTORC1 and mTORC2 substrates in DEPTOR knockdown cells. (E, F) Protein expression of mTORC1 substrates in PRAS40 knockdown cells. N = 6. (*) for t-test p < 0.05.