Research Paper Volume 14, Issue 6 pp 2678—2694

piRNA-6426 increases DNMT3B-mediated SOAT1 methylation and improves heart failure


Figure 7. SOAT1 interference alleviates hypoxia-induced dysfunction of rat cardiomyocytes. Hypoxia-induced cardiomyocytes were transfected with sh-SOAT1 or negative control scrambled shRNA for 48 h. (A, B) Western blotting was used to detect the expression level of SOAT1 protein. (C) MTT assay was used to test cell viability. (D, E) Flow cytometry was used to detect cell apoptosis. (F) The production of ROS was analyzed with DCFH-DA. (G) The LDH activity was detect with a LDH ELISA kit. (H) D-(2-3H)-glucose uptake assay was used to perform glucose uptake on fully fused rat cardiomyocytes. (I, J) ELISA kits were used to detect the secretion of IL-1β and TNF-α. Values were expressed as mean ± SEM. *P<0.05, n=6.