Research Paper Volume 14, Issue 6 pp 2695—2719

Figure 1. Generation of the experimental animals. Representative graphics for the characterization of the animal models. CD-1 mice fed for 12 months with standard food ad libitum (first stage) were separated into animals fed standard food ad libitum and animals fed an HCD ad libitum for a period of 17 weeks (second stage). Some animals were subjected to CR for a period of 13 weeks before sacrifice (third stage). The experimental groups included mice fed a 3.1 kcal/kg diet and subjected to CR (the 3.1CR group) and animals with an HCD background subjected to CR (the 5.5CR group).