Research Paper Volume 14, Issue 7 pp 3049—3069

Figure 1. Profiling of circRNAs in old endothelial cells (OECs) and young endothelial cells (YECs). (A) The circle shows the distribution and expression of differentially expressed circRNAs and mRNAs on mouse chromosomes. The outer, middle and inner layers represent the mouse chromosomes, mRNAs and circRNAs, respectively. Red represents upregulation, and green represents downregulation in YECs. (B) The distribution of circRNAs of different lengths in YECs compared with OECs. (C) Different mouse genomic sources of differential circRNAs. (D) Volcano map of circRNAs in YECs compared with OECs. Red represents upregulation, green represents downregulation, and blue represents no significant difference. (E) The heat map shows the differentially expressed circRNAs in OECs and YECs. The red bars represent upregulation, and the blue bars represent downregulation. (F) qRT-PCR shows ten of the most significant differences circRNAs expression in YECs compared with OECs; the expression was normalized to that of GAPDH (n=3). (G) qRT-PCR analysis of circGSE1 expression in the lower limb gastrocnemius muscles of mice with femoral artery ligation ischaemia; expression was normalized to that of GAPDH (n=3). (The data are expressed as the mean ± SD, *P < 0.05, **P < 0.01 versus the negative control).