Research Paper Volume 14, Issue 7 pp 3049—3069

Figure 3. CircGSE1 promotes the proliferation, migration and tube formation of MAECs in vitro. (A) Schematic diagram of the circGSE1 siRNA and overexpression vectors. (B) qRT-PCR detection of YECs demonstrating successful knockdown of circGSE1; however, the linear GSE1 RNA expression was not altered significantly (n=3). (C) qRT-PCR detection demonstrating successful circGSE1 overexpression in OECs, while the linear GSE1 RNA expression was not changed significantly (n=3). (D) Wound healing assay of OECs treated with vector and OE-circGSE1, YECs treated with NC and si-circGSE1 (n=3, scale bar, 200 μm). (E) Tube formation assay of OECs treated with vector and OE-circGSE1, YECs treated with NC and si-circGSE1 (n=3, scale bar, 200 μm). (F) Migration assay of OECs treated with vector and OE-circGSE1, YECs treated with NC and si-circGSE1 (n=3, scale bar, 200 μm). (G) EdU assay of OECs treated with vector and OE-circGSE1, YECs treated with NC and si-circGSE1 (n=3, scale bar, 200 μm). (n=5, scale bar, 100 μm). (The data are expressed as the mean ± SD, *P < 0.05, **P < 0.01 versus the negative control).