Research Paper Volume 14, Issue 7 pp 3049—3069

Figure 6. NRP1 is the downstream target of miR323-5p. (A, B) Western blot analysis of the NRP1 expression in OECs and YECs. (C) qRT-PCR analysis of the NRP1 mRNA expression in OECs and YECs. (D) Western blot analysis of the NRP1 expression in OECs transfected with negative control and miR323-5p inhibitor, YECs transfected with negative control and miR323-5p mimics. (E) qRT-PCR analysis of the NRP1 mRNA expression in YECs transfected with negative control and miR323-5p mimics, OECs transfected with negative control and miR323-5p inhibitor. (F) Schematic diagrams of the circGSE1-WT and circGSE1-mutant dual-luciferase reporter vectors. (G) Dual-luciferase reporter assays showed that the circGSE1 wt 3’UTR but not the mut 3’UTR was downregulated by miR-323-5p mimics or upregulated by the miR-323-5p inhibitor. (H, I) WB analyses of OECs treated with the vector, OE-circGSE1 or OE-circGSE1 + miR323-5p mimic and YECs treated with the NC, si-circGSE1 or si-circGSE1+miR323-5p inhibitor. (The data are expressed as the mean ± SD, *P < 0.05, **P < 0.01 versus the negative control).