Research Paper Volume 14, Issue 7 pp 3049—3069
Circular RNA circGSE1 promotes angiogenesis in ageing mice by targeting the miR-323-5p/NRP1 axis
Figure 7. CircGSE1 regulates the angiogenic ability of MAECs via the NRP1. (A) Wound healing assay of OECs treated with vector, the OE-NRP1 or the OE-NRP1 + circGSE1 siRNA and YECs treated with the NC, NRP1-siRNA or NRP1-siRNA +OE-circGSE1. The graph indicates the quantification of the wounded area 24 hours after scratching (n=3, scale bar: 200 μm). (B) Tube formation assay of OECs treated with the vector, the OE-NRP1 or the OE-NRP1 + circGSE1 siRNA and YECs treated with the NC, NRP1-siRNA or NRP1-siRNA +OE-circGSE1 (n=3, scale bar, 200 μm). (C) Migration assay of OECs treated with the vector, the OE-NRP1 or the OE-NRP1 + circGSE1 siRNA and YECs treated with the NC, NRP1-siRNA or NRP1-siRNA +OE-circGSE1 (n=5, scale bar, 200 μm). (D) EdU assay of OECs treated with the vector, the OE-NRP1 or the OE-NRP1 + circGSE1 siRNA and YECs treated with the NC, NRP1-siRNA or NRP1-siRNA +OE-circGSE1 (n=5, scale bar, 200 μm). (The data are expressed as the mean ± SD, *P < 0.05 **P < 0.01 versus the negative control).