Research Paper Volume 14, Issue 7 pp 3143—3154
Elevated lncRNA MIAT in peripheral blood mononuclear cells contributes to post-menopausal osteoporosis
Figure 2. lncMIAT upregulates inflammatory cytokine secretion in PBMCs. (A) Transfection efficacy of lncMIAT and siMIAT was measured in PBMCs by RT-qPCR assays. GAPDH was used as control. (B–D) ELISA assays of IL-6, TNF-α and IL-1β were performed with the cultured medium of transfected cells. Elevated lncMIAT expression increased inflammatory cytokine secretion in PBMCs, while lncMIAT silencing reduced inflammatory cytokine levels. *, p < 0.05.