Research Paper Volume 14, Issue 7 pp 3143—3154

Figure 4. lncMIAT directly binds with miR-216a. (A) Bioinformatic analysis was performed for the miRNAs which were potentially interacted with lncMIAT and p38MAPK. VENN analysis was performed for the miRNA targets in common. (B, C) The expression levels of miR-216a and miR-128 was compared between p38MAPK high and low group in our cohort (n = 40). (D) LncBase v.2 analysis predicted the binding site of lncMIAT and miR-216a. Wild type and mutant miR-216a were prepared as the sequence shown in diagram. (E) PBMCs were co-infected with lncMIAT and miR-216a-wt or miR-216a-mut. Relative levels of lncMIAT and GAPDH were analyzed in the miR-216a pulled down pellet with RT-qPCR assays. (F) RT-qPCR assays were performed for the miR-216a expression in lncMIAT overexpressing or silencing cells. Negative correlation was observed between lncMIAT and miR-216a expression. (G) lncMIAT expression levels were detected in miR-216a negative control (NC) or mimics infected cells, which were analyzed with RT-qPCR assays. *, p < 0.05.