Research Paper Volume 14, Issue 7 pp 3216—3232
Trehalose inhibits ferroptosis via NRF2/HO-1 pathway and promotes functional recovery in mice with spinal cord injury
Figure 4. Trehalose inhibits the production of ROS and lipid peroxides after SCI. (A, B) DHE staining of spinal cord tissue on day 3 after SCI and fluorescence quantitative analysis; (C) GSH measurement; (D) MDA measurement; (E–G) Western blot to detect the expression of Acsl4 and Gpx4 protein and quantitative protein analysis; (H, I) PCR to measure the expression of Acsl4 mRNA and Gpx4 mRNA. *p < 0.05 compared with the Sham group, #p < 0.05 compared with the SCI group.