Research Paper Volume 14, Issue 7 pp 3276—3292

Figure 2. Acquisition of characteristic genes associated with immune subtypes. (A) After dimension reduction through PCA, we found that the cells were clustered into 9 clusters. It can be seen that the expression of the first 10 genes in each cluster is significantly higher than that in other clusters. (B, C) We can find the distribution of these 9 clusters, and the cluster labels corresponding to immune cells are 5,6,7, and 8. Cells can be divided into immune cells and non-immune cells. (D–F) We then explored the differentiation trajectory of immune and non-immune cells. With the time change of cell differentiation, that is, cells differentiate from the deeper blue branches to the lighter blue branches, the differentiation states of immune cells and non-immune cells are different, and the differentiation time of non-immune cells may be earlier. Then we obtained 526 genes related to immune subtypes by using the Findmarkers function.