Research Paper Volume 14, Issue 7 pp 3313—3324
SNP rs322931 (C>T) in miR-181b and rs7158663 (G>A) in MEG3 aggravate the inflammatory response of anal abscess in patients with Crohn’s disease
Figure 5. Sequence analysis indicated the binding of miR-181b to the 3’ UTR of MEG3 (A) and TNF-α (B), respectively. And the luciferase activity of wild-type MEG3 (C) as well as TNF-α (D) was suppressed by miR-181b in THP-1 cells, thus verifying that miR-181b could suppress the expression of MEG3 and TNF-α through binding to their 3’ UTR (* P value < 0.05 vs. NC group; 3 biological repeats).