Research Paper Volume 14, Issue 8 pp 3400—3415

Figure 4. Functions of Circ_0000423 in OA by targeting miRNA-27b-3p expression as a ceRNA. (A) The mRNA expression of Circ_0000423; (B) The mRNA expression of miRNA-27b-3p; (C) The mRNA expression of MMP-13; (D) The mRNA expression of COL-2; (E) The proteins expression of MMP-13 and COL-2; (F, G) The relative protein expression of COL-2 and MMP-13are demonstrated with bar graphs. (H) The relative protein expression of COL-2 and MMP-13 are demonstrated with Immunofluorescence analysis. (I, J) The relative fluorescence expression of COL-2 and MMP-13 are demonstrated with bar graphs. P-circ: overexpression of Circ_0000423; si-circ-1-3: siRNA against Circ_0000423; Data are presented as mean ± SEM. ^*P < 0.05 compared with the NC group, ^#P < 0.05 compared with the p-circ group, ^&P < 0.05 compared with the si-circ-1 group.