Research Paper Volume 14, Issue 8 pp 3529—3539
NRIP1 aggravates lung injury caused by Pseudomonas aeruginosa in mice by increasing PIAS1 ubiquitination
Figure 1. Effects of NRIP1 on PA treated TC-1 cells. TC-1 cells were treated with PA011, and the pcDNA-NRIP1 or si-NRIP1 was transfected into cells, respectively. (A) The protein expression of NRIP1 was analyzed by Western botting. (B) MTT assay was used to analyzed the viability of cells. (C) Apoptosis of cells were detected by flow cytometry. (D, E) Relative content of TNF-α and IL-1β were analyzed by ELISA kits. GAPDH was used as an invariant internal control for calculating protein-fold changes. N = 6, **P < 0.01.