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Research Paper Volume 14, Issue 8 pp 3529—3539

NRIP1 aggravates lung injury caused by Pseudomonas aeruginosa in mice by increasing PIAS1 ubiquitination

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Figure 2. UBE2I interacts with NRIP1. (A) The online biological database was used to predict the interaction relationship NRIP1 between and UBE2I (https://inbio-discover.intomics.com/map.html). (B) The pcDNA-NRIP1 or si-NRIP1 was transfected into TC-1 cells, respectively, and the protein expression of UBE2I was detected by Western blotting. (C) The binding relationship of NRIP1 and UBE2I was validated by co-immunoprecipitation assay. GAPDH was used as an internal reference. N = 6, *P < 0.01.