Research Paper Volume 14, Issue 8 pp 3529—3539
NRIP1 aggravates lung injury caused by Pseudomonas aeruginosa in mice by increasing PIAS1 ubiquitination
Figure 3. NRIP1 affects PA treated TC-1 cells by positively regulating UBE2I expression. The PA011-treated TC-1 cells were transfected with si-NRIP1 alone or together with pcDNA-UBE2I. (A) The protein expression of UBE2I was analyzed by Western botting. (B) MTT assay was used to analyzed the viability of cells. (C) Apoptosis of cells were detected by flow cytometry. (D, E) Relative contents of TNF-α and IL-1β were analyzed by ELISA kits. GAPDH was used as an internal reference. N = 6, *P < 0.01.