Research Paper Volume 14, Issue 8 pp 3529—3539

Figure 4. PIAS1 interacts with UBE2I. (A) The online biological database was used to predict the interaction relationship UBE2I between and PIAS1 (https://inbio-discover.intomics.com/map.html). (B) The binding relationship of UBE2I and PIAS1 was validated by co-immunoprecipitation assay. (C) Co-immunoprecipitation assay was used to validate the binding relationship of NRIP1, UBE2I and PIAS1. (D) The pcDNA-UBE2I or si-UBE2I was transfected into cells, and the protein expression of UBE2I and PIAS1was analyzed by Western botting. (E) The cells were transfected with pcDNA-NRIP1 alone or together with si-UBE2I, and the protein expression of PIAS1was analyzed by Western botting. GAPDH was used as an internal reference. N = 6, ^*P < 0.01.