Research Paper Volume 14, Issue 8 pp 3529—3539
NRIP1 aggravates lung injury caused by Pseudomonas aeruginosa in mice by increasing PIAS1 ubiquitination
Figure 5. UBE2I accelerates PIAS1 ubiquitination for degradation. (A) The si-UBE2I was transfected into cells and the ubiquitination level of PIAS1 was detected by co-immunoprecipitation assay. (B) The pcDNA-UBE2I was transfected into cells and the ubiquitination level of PIAS1 was detected by co-immunoprecipitation assay. (C) UBE2I was overexpressed alone or simultaneously with NRIP1, and the ubiquitination level of PIAS1 was detected by co-immunoprecipitation assay. GAPDH was used as an internal reference. N = 6, *P < 0.01.