Research Paper Volume 14, Issue 8 pp 3529—3539

Figure 7. Interference with NRIP1 expression attenuates lung injury in PA infected mice. A total of 18 mice randomly divided into control group (injection of the same volume of 0.9% sterile saline solution as the PA011), PA group (intratracheally instilled with 50 μL of PA011) and si-NRIP1 group (PA treated mice were injected intraperitoneally with 20 nM si-NRIP1 once daily), with six mice in each group. (A) The protein expression of NRIP1, UBE2I and PIAS1 in lung tissues was analyzed by Western botting. (B) Relative content of TNF-α and IL-1β in lung tissues were analyzed by ELISA kits. (C) Representative images of HE staining of lung tissues. (D, E) Total lung capacity (TLC) and tissue elastance (H) were assessed using FlexiWare software. (F) The ratio of lung wet-to-dry weight was calculated. GAPDH was used as an internal reference. ^*P < 0.01.