Research Paper Volume 14, Issue 8 pp 3652—3665

Figure 6. miR-569 directly targeted NUSAP1. (A) Oncomine database showing NUSAP1 mRNA expression level in PC and normal tissues. (B, C) Kaplan-Meier overall survival and disease-free survival curves for patients with PC stratified by high and low expression of NUSAP1. (D) Putative miR-569 target sequence in wild-type (WT) and mutated (MUT) 3’UTR of NUSAP1 was generated as indicated. (E) Relative luciferase activity of NUSAP1 3’UTR co-transfected with the indicated reporters and miR-569 mimic oligonucleotides. (F) Western blot assay demonstrated NUSAP1 protein level after over-pression of miR-569. (* p < 0.05, ** p < 0.01, n = 5, Student’s t-test, means ± 95% CI).