Research Paper Volume 14, Issue 8 pp 3666—3686
LncRNA136131 suppresses apoptosis of renal tubular epithelial cells in acute kidney injury by targeting the miR-378a-3p/Rab10 axis
Figure 1. I/R Induced the expression of lncRNA136131 in BUMPT cells. BUMPT cells were subjected to ischemic (I, 2 hours)/reperfusion (R, 0, 2, and 4 hours) treatment. (A) RT-qPCR analysis of the expression of lncRNA136131. (B) The immunoblot analysis of the expression of caspase 3, cleaved-caspase3, Bax, p53 and Blc-2. (C). Densitometric measurement of western blot bands of them. (D) The RNA-FISH detection of Intracellular localization of lncRNA136131 in BUMPT cells. U6 and 18S were used as control of nucleus and cytoplasm markers, respectively. Data are expressed as mean ± SD (n = 6). *p < 0.05, I/R group versus control group.