Research Paper Volume 14, Issue 8 pp 3666—3686
LncRNA136131 suppresses apoptosis of renal tubular epithelial cells in acute kidney injury by targeting the miR-378a-3p/Rab10 axis
Figure 4. lncRNA136131 inhibits the expression and activity of miR-378a-3p. (A) Sequence alignment analysis of the complementary strand of lncRNA136131 and miR-378a-3p. (B) Detection of luciferase activities after co-transfection with miR-378a-3p plus with lncRNA136131-WT or lncRNA136131-MUT. (C) The RNA-FISH detection of Intracellular co-localization of lncRNA136131 and miR-378a-3p in BUMPT cells and mice kidney treated with or without I/R injury. (D, E) RT-qPCR analysis of miR-378a-3p expression. Data are expressed as mean ± SD (n = 6). *p < 0.05, I/R with Scramble group versus Scramble group; #p < 0.05, I/R with lncRNA136131 siRNA or plasmid group versus I/R with Scramble group, or lncRNA136131 WT/miR-378a-3p mimic versus other groups.