Research Paper Volume 14, Issue 8 pp 3666—3686

Figure 5. Overexpression of miR-378a-3p aggravated the I/R-Induced expression levels of cleved-caspase3 in BUMPT cells. BUMPT cells were transfected with 100 nM miR -378a-3p mimics or scramble and then treated with or without I(2 hours)/R(2 hours) injury. (A) The mRNA expression levels of miR-378a-3p were detected by real-time qPCR. (B) FCM analysis of BUMPT cells apoptosis. (C) Analysis of apoptosis rate (%). (D) The immunoblot analysis of caspase 3, cleaved-caspase3, Bax, p53 and Blc-2. (E) The gray analysis between them. Data are expressed as mean ± SD (n = 6). ^*p < 0.05, I/R with scramble group versus scramble group; ^#p < 0.05, I/R with miR-378a-3p mimics group versus I/R with scramble group.