Research Paper Volume 14, Issue 8 pp 3666—3686
LncRNA136131 suppresses apoptosis of renal tubular epithelial cells in acute kidney injury by targeting the miR-378a-3p/Rab10 axis
Figure 7. Knockdown Rab10 aggravates I/R-induced BUMPT cells apoptosis and the expression of cleaved-caspase3. BUMPT cells were transfected with siRNA Rab10 (100 nM), and then treated with I(2 hours)/R(2 hours) injury. (A) RT-qPCR analysis of the expression of Rab10. (B) Immunoblot analysis of Rab10 and β-tubulin at indicated time points. (C) Densitometric analysis of proteins signals. (D) FCM analysis of BUMPT cells apoptosis. (E) The apoptosis rate (%). (F) Immunoblot analysis of caspase 3, cleaved-caspase3, Bax, Blc-2, p53 and Rab10. (G) Densitometric analysis of proteins signals. Data are expressed as mean ± SD (n = 6). *p < 0.05, I/R with scramble group or I/R group versus scramble group or control group; #p < 0.05, I/R with siRNA Rab10 group versus I/R with scramble group.