Research Paper Volume 14, Issue 8 pp 3666—3686
LncRNA136131 suppresses apoptosis of renal tubular epithelial cells in acute kidney injury by targeting the miR-378a-3p/Rab10 axis
Figure 8. Overexpression of Rab10 attenuated I/R-induced BUMPT cells apoptosis and the expression of cleaved-caspase3. Rab10 plasmid (100 nM) was transfected into BUMPT cells, and then treated with I(2 hours)/R(2 hours). (A) FCM analysis of BUMPT cells apoptosis. (B) The apoptosis rate (%). (C) Immunoblot analysis of caspase 3, cleaved-caspase3, Bax, Blc-2, p53 and Rab10. (D) Densitometric analysis of proteins signals. Data are expressed as mean ± SD (n = 6). *p < 0.05, I/R with scramble group or I/R group versus scramble or control group ; #p < 0.05, I/R with Rab10 plasmid group versus I/R with scramble group.