Research Paper Volume 14, Issue 8 pp 3666—3686

Figure 9. LncRNA136131 knockdown aggravated the I/R-Induced BUMPT cells apoptosis and the expression of cleaved caspase3, this was reversed by miR-378a-3p inhibitor. BUMPT cells were co-transfected with siRNA lncRNA136131 (100 nM) plus with or without anti-miR-378a-3p, and then treated with I(2 hours)/R(2 hours) injury. (A) RT-qPCR analysis the expression of lncRNA136131. (B) Real-time qPCR analysis of miR-378a-3p expression. (C) FCM analysis of BUMPT cells apoptosis. (D) Cell apoptosis rate (%). (E) Immunoblot analysis of caspase 3, cleaved-caspase3, Bax, Blc-2, p53 and Rab10. (F) Densitometric measurement of protein signals. Data are expressed as mean ± SD (n = 6). ^*p < 0.05, I/R with scramble group versus scramble group, ^#p < 0.05, I/R with lncRNA136131 siRNA group versus I/R with scramble group; ^Δp < 0.05, I/R with lncRNA136131 plus anti-miR-378a-3p group versus I/R with lncRNA136131 siRNA group.