Research Paper Volume 14, Issue 8 pp 3365—3386
Wild type and gain of function mutant TP53 can regulate the sensitivity of pancreatic cancer cells to chemotherapeutic drugs, EGFR/Ras/Raf/MEK, and PI3K/mTORC1/GSK-3 pathway inhibitors, nutraceuticals and alter metabolic properties
Figure 4. Effects of GSK-3 inhibitors and the mTORC1 blocker rapamycin on the growth of MIA-PaCa-2 + WT-TP53 and MIA-PaCa-2 + pLXSN cells. The effects of the BIO GSK-3 inhibitor (A), the SB415286 GSK-3 inhibitor (B), the CHIR99021 GSK-3 inhibitor (C) and the mTORC1 blocker rapamycin (D) on MIA-PaCa-2 + pLXSN cells (solid red squared) and MIA-PaCa-2 + WT-TP53 cells (solid blue circles) were examined by MTT analysis. The MIA-PaCa-2 + WT-TP53, and MIA-PaCa-2 + pLXSN cells in each panel were all examined at the same time period. These experiments were repeated and similar results were obtained. Statistical analyses were performed by the T test on the means and standard deviations of various treatment groups. ***P < 0.0001.