Research Paper Volume 14, Issue 9 pp 3728—3756

Figure 5. Igfbp3 expression in young versus middle-aged NMR keratinocytes. (A) Expression levels for Igfbp3 gene onto Uniform Manifold Approximation and Projection (UMAP). Note that its expression is restricted to basal stem and proliferative cell clusters BSC1-2-3 and BPSC. (B) Expression levels for Igfbp3 gene onto UMAP for MA (middle-aged) and Y (young) NMR. The dotted circle delimits BSC2 cluster. (C) Violin plots of Igfbp3 genes expressed by MA (red) and Y (blue) epidermal cells. (D) IGFBP3 staining photomicrographs on sections of MA and Y NMR back skin. Cells were stained with Alexa 546 fluorochromes. Negative controls were performed in parallel with the samples substituting the primary antibody with the equivalent isotype. Scale bar = 50 μm. Histogram showing the quantification of nuclear IGFBP3 expression in both age group. Bars: SEM. *represents differences between the age groups. ****p≤0.0001 in Student’s t-test. n=4 animals per group.