Research Paper Volume 14, Issue 9 pp 3941—3955

Elucidating the anti-aging mechanism of Si Jun Zi Tang by integrating network pharmacology and experimental validation in vivo


Figure 5. SJZT can improve the aging-related phenotype of aging mice. (A) Body weight of mice after SJZT or distilled water treatment. (B) Representative photos of the old mice and after SJZT treatment with considerable differences in skin and in hair. And μCT of the isolated shin bones to assess bone porosity. (C) The protein levels of P16, P21 and P53 determined by Western blots in mice liver tissue. (D) The mRNA levels of p16 and IL6 determined by qPCR in mice Liver tissue. (E, F) The protein expression levels of liver tissues were detected by western blot analysis. Data represent the means ± standard deviation. *P<0.05, **P<0.01 or ***P<0.001 (n=3).