Research Paper Volume 14, Issue 9 pp 4000—4013

Figure 2. PAM treatment can induce ROS accumulation and MMP loss in A549 cells leading to cell death. (A) Knockdown of PRDX5 after transfection with shPRDX5 was verified in A549 cells via western blotting using PRDX5 antibodies. (B) Changes in expression levels of peroxide reductase family members in the A549 PRDX5 knockdown cell line after PAM treatment. (C) Comparison of cell viability between the shPRDX5 and control groups. (D and E) Imaging of shPRDX5-transfected A549 cells and control A549 cells exposed to PAM and stained with JC-1. (F and G) Relative fluorescence intensity of Mito-SOX staining. The data are presented as the mean ± standard deviation of three independent experiments. Significant differences are indicated at ^*p < 0.05; ^**p < 0.01; ^***p < 0.001 vs. the control. Con, control.