Research Paper Volume 14, Issue 9 pp 4000—4013
Knockdown of Peroxiredoxin V increased the cytotoxicity of non-thermal plasma-treated culture medium to A549 cells
Figure 4. PAM induces ROS accumulation and MMP loss in A549 cells, leading to cell death. (A) Images of A549 cells exposed to PAM and stained with Annexin V. (B) Images of A549 cells exposed to PAM and stained with DHE. Relative fluorescence intensity of Annexin V staining. (C) Relative fluorescence intensity of Annexin V staining. (D) Relative fluorescence intensity of DHE staining. (E–K): Western blot analysis was performed to check the expression of apoptosis-related proteins. The data are presented as the mean ± standard deviation of three independent experiments. Significant differences are indicated at *p < 0.05; **p < 0.01; ***p < 0.001 vs. the control. Con, control.