Research Paper Volume 14, Issue 9 pp 4000—4013

Knockdown of Peroxiredoxin V increased the cytotoxicity of non-thermal plasma-treated culture medium to A549 cells


Figure 5. NAC addition eliminated the inhibitory effect of PAM on the malignant behavior of A549 cells after PRDX5 knockdown. (A) Images on the first day after the cancer cells attached to the bottom of the plates; cells were pre-treated with NAC. The culture medium was removed, and PAM and NAC were added. (B) Colony formation assay performed to detect the colony formation ability of A549 cells after PAM exposure for 7 days. (C) Images of wound-healing assays. (D) Quantification of wound-healing area. The data are presented as the mean ± standard deviation of three independent experiments. Significant differences are indicated at *p < 0.05; **p < 0.01; ***p < 0.001 vs. the control. Con, control.