Research Paper Volume 14, Issue 9 pp 4000—4013

Figure 7. Effects of NAC treatment on intracellular ROS content in PRDX5 knockdown A549 lung cancer cells. (A) Images of A549 cells exposed to PAM and stained with Annexin V after NAC treatment. (B) Relative fluorescence intensity of Annexin V staining. (C) Images of A549 cells exposed to PAM and stained with DHE after NAC treatment. (D) Relative fluorescence intensity of DHE staining. (E) Flow cytometric analysis of intracellular ROS production in A549 cells using DHE staining. (F) Western blotting of apoptosis-related proteins, including AKT, pro-caspase 3, and PARP in A549 cells after treatment with NAC. Quantified data are presented as the mean ± standard deviation of three independent experiments. Significant differences are indicated at ^*p < 0.05; ^**p < 0.01; ^***p < 0.001 vs. the control. Con, control.