Research Paper Volume 14, Issue 10 pp 4336—4356
Identification and validation of aging-related genes in COPD based on bioinformatics analysis
Figure 10. Prediction of miRNAs that could regulate HIF1A, CDKN1A, MXD1 and SOD2. (A) Venn diagram of miRNAs predicted to target CDKN1A, HIF1A, MXD1 and SOD2. (B) The predicted interactions of hsa-miR-519d-3p with CDKN1A, HIF1A, MXD1 and SOD2. (C) qRT-PCR expression analysis of hsa-miR-519d-3p in PBMCs from COPD and healthy volunteers; data are presented as 2(-ΔCт) relative to U6. (D) Expression analysis of hsa-miR-519d-3p in Beas-2B cell following transfection with hsa-miR-519d-3p mimic. (E) Expression of CDKN1A, HIF1A, MXD1 and SOD2 in Beas-2B cell following transfecting with hsa-miR-519d-3p mimic; data are presented as 2(-ΔΔCт) relative to GAPDH. *P <0.05; **P <0.01.