Research Paper Volume 14, Issue 10 pp 4336—4356
Identification and validation of aging-related genes in COPD based on bioinformatics analysis
Figure 11. Regulatory interactions between hsa-miR-519d-3p and CDKN1A, HIF1A, MXD1 and SOD2. (A) Luciferase reporter assay for detection of the interaction of hsa-miR-519d-3p with 4 candidate genes. (B) Results of luciferase reporter assay demonstrate that hsa-miR-519d-3p directly binds to CDKN1A. (C) Western blot analysis of the effect of hsa-miR-519d-3p on CSE-induced aging-related proteins. *P <0.05; **P <0.01.