Research Paper Volume 14, Issue 10 pp 4376—4389

Figure 2. has_circ_0069313 correlated with PDL1 level in OSCC samples. (A) Samples were collected and subjected to IHC using the CD8 antibody. The representative images were shown with a 200× and 400× magnification. (B) The relative has_circ_0069313 level and CD8 IHC score were collected and regression analysis was applied, R = 0.41, p < 0.001. (C) Samples were collected and subjected to IHC using the PDL1 antibody. The representative images were shown with a 200× and 400× magnification. (D) The relative has_circ_0069313 level and PDL1 IHC score were collected and regression analysis was applied, R = 0.83, p < 0.001. (E) Samples were collected and subjected to immunoblot, PDL1 was detected as indicated. (F) has_circ_0069313 was knocked down using junction specific shRNAs and overexpression cell was established using the OV plasmid. has_circ_0069313 was detected and normalized. (Student’s two-tailed paired test, ^***p < 0.001). (G) The relative PDL1 RNA level in cells with indicated modifications. (H) Immunoblot of PDL1 in cells with indicated modifications.