Research Paper Volume 14, Issue 10 pp 4376—4389

Figure 6. has_circ_0069313 correlated with Treg infiltration. (A) Samples were collected and subjected to IHC using the CD25 antibody. The representative images were shown with a 200× and 400× magnification. (B) The relative has_circ_0069313 level and CD25 IHC score were collected and regression analysis was applied, R = 0.61, p < 0.001. (C) The graphic illustration of coculture strategy and exosome treatment strategy. (D) Treg cells were treated with OSCC derived exosomes or co-cultured with OSCC. The Treg cells were collected and subjected to qRT-PCR, relative has_circ_0069313 was measured (Student’s two-tailed paired test, ^***p < 0.001). (E) Relative miR-325-3p was detected in Treg cells with indicated treatments (Student’s two-tailed paired test, ^***p < 0.001). (F) Immunoblot was applied for Foxp3 in Treg cells with indicated treatments.