Priority Research Paper Volume 14, Issue 10 pp 4195—4210
Long-term treatment with chloroquine increases lifespan in middle-aged male mice possibly via autophagy modulation, proteasome inhibition and glycogen metabolism
Figure 3. CQ treatment caused an increase in the LC3-II/LC3-I ratio and p62 levels in the liver and heart homogenates. In our toxicity study, there was a progressive, significant increase in the LC3-II/LC3-I ratio in the liver (A, B). The increase in the LC3-II/LC3-I ratio was paralleled by an increase in the expression level of p62 in the liver (C). The increase in the LC3-II/LC3-I ratio with increasing chloroquine concentration in drinking water was also significant, albeit less obvious in the heart homogenates (D, E). The increase in the LC3-II/LC3-I ratio was paralleled by an increase in the expression level of p62 in the heart (F) homogenates. Data are mean ± SD values. N = 5 for each group.