Research Paper Volume 14, Issue 10 pp 4220—4246
Senescence-associated morphological profiles (SAMPs): an image-based phenotypic profiling method for evaluating the inter and intra model heterogeneity of senescence
Figure 3. Phenotypic profiling of senescence via standard score profile generation. (A) Schematic overview of data processing proceeding standard-score generation for each senescence model (P: proliferating condition, S: Senescence condition). (B–E) Standard-score profile heatmaps for oncogene-induced senescence (OIS), paracrine senescence (Paracrine), HMF replicative senescence (HMF RS) and HDF replicative senescence (HDF RS) models. Y-axis comprises 62 morphological features (Red = positive modulation, Blue = negative modulation), White = no change). Proliferating conditions: vector induction (Vec_Ind), vector conditioned media (Vec_CM), HMF early proliferating (HMF_EP) and HDF early proliferating (HDF_EP). Senescence conditions: OIS induction (OIS_Ind), OIS conditioned media (OIS_CM), HMF deep senescence (HMF_DS), HDF deep senescence (HDF_DS). (F) Summary standard-score profile heat map and hierarchical clustering of all proliferating and senescence conditions. Y-axis comprises 62 morphological features.