Research Paper Volume 14, Issue 10 pp 4220—4246
Senescence-associated morphological profiles (SAMPs): an image-based phenotypic profiling method for evaluating the inter and intra model heterogeneity of senescence
Figure 7. Assessment of senescence morphology in palbociclib treated human tumour xenografts. (A and B) Analysis mask generation to define p21 positive (red) and negative (blue) nuclei in control and palbociclib treated xenografts. Blue stain = Counterstain, Brown stain = p21. Scale bar = 200 μm. (C) Percentage p21 positivity of nuclei in control and palbociclib treated xenografts. (D) Mean standard score profiles of control and palbociclib samples. Y-axis comprises 27 morphological features (Red = positive modulation, Blue = negative modulation), White = no change). (E and F) Z-score and standard score profiles of p21 positive and negative cells in control and palbociclib treated xenografts. Y-axis comprises 27 morphological features (Red = positive modulation, Blue = negative modulation), White = no change). N = 3.