Research Paper Volume 14, Issue 10 pp 4445—4458
A real-time pluripotency reporter for the long-term and real-time monitoring of pluripotency changes in induced pluripotent stem cells
Figure 4. EGFP expression associated with Oct4 and Sox2 expression during the EB-mediated differentiation of Oct4-EGFP miPSCs. (A) The undifferentiated/differentiated cell state was assessed through GFP assay by flow cytometry (FCM) at 7th day, 14th day and 21st day after EB-mediated differentiation. (B) qRT-PCR analysis for the expression of ESC-specific transcription factors (i.e., Oct4 and Sox2) in Oct4-EGFP miPSCs and differential EBs.