Research Paper Volume 14, Issue 10 pp 4486—4499
Long noncoding RNA KCNQ1OT1 inhibits osteoclast differentiation by regulating the miR-128-3p/NFAT5 axis
Figure 3. KCNQ1OT1 directly targets miR-128-3p. (A) StarBase, miRwalk and TargetScan databases showing that miR-128-3p might be a potential target of KCNQ1OT1. (B) Relative expression of miR-128-3p in RAW 264.7 cells transfected with pcDNA-KCNQ1OT1, si-KCNQ1OT1, or corresponding control vectors. (C) StarBase database was used to predict the binding site between KCNQ1OT1 and miR-128-3p. Dual-luciferase reporter assays on HEK-293T cells transfected with agomiR-NC or agomiR-128-3p in wild-type (WT) and mutant-type (MUT) groups. ***P < 0.001, ns: not significant.